human circrna array v2 (4 × 180k)  (CapitalBio Corporation)

Journal: EBioMedicine

Article Title: Potential Diagnostic Power of Blood Circular RNA Expression in Active Pulmonary Tuberculosis

doi: 10.1016/j.ebiom.2017.12.007

Figure Lengend Snippet: The experimental scheme of the study. The discovery cohort was composed of two TB patients and two age- and gender-matched healthy controls: one young male case-control pair and one senior female case-control pair. PBMC circRNA expression in the discovery cohort was profiled by both rRNA-depleted RNA-seq and a circRNA expression microarray. We aggregated circRNAs into pathway-level mechanisms using KEGG pathway definitions. Pathways with significant circRNA dysregulation were prioritized by performing a paired Wilcoxon signed-rank test between paired control and TB samples. A molecular signature was developed based on the dysregulated circRNAs within the prioritized pathways. This circRNA signature was further validated by qRT-PCR in a validation cohort with 11 healthy controls and 10 TB patients.

Article Snippet: The CapitalBio Technology Human CircRNA Array v2 (4 × 180K) was used to quantify circRNA expression.

Techniques: Control, Expressing, RNA Sequencing, Microarray, Quantitative RT-PCR, Biomarker Discovery

Journal: EBioMedicine

Article Title: Potential Diagnostic Power of Blood Circular RNA Expression in Active Pulmonary Tuberculosis

doi: 10.1016/j.ebiom.2017.12.007

Figure Lengend Snippet: Landscape of PBMC circRNA expression. (a) A Venn diagram of the parental genes with circRNA transcripts among PBMCs, platelets, RBCs, and whole blood. (b) Distribution of log 2 -transformed TPM values of circular transcripts in PBMCs, platelets, RBCs, and whole blood. (c) The fraction of RNA-seq reads from coding linear, non-coding linear, and circular transcripts. (d) The cumulative distribution of TPM values of circular transcripts. A significantly increased circRNA TPM was observed in the TB patients compared with the healthy controls. The P -values were computed by the Kolmogorov-Smirnov test.

Article Snippet: The CapitalBio Technology Human CircRNA Array v2 (4 × 180K) was used to quantify circRNA expression.

Techniques: Expressing, Transformation Assay, RNA Sequencing

Journal: EBioMedicine

Article Title: Potential Diagnostic Power of Blood Circular RNA Expression in Active Pulmonary Tuberculosis

doi: 10.1016/j.ebiom.2017.12.007

Figure Lengend Snippet: The KEGG pathways enriched by upregulated circRNAs. A paired Wilcoxon test was applied to identify the KEGG pathways enriched by dysregulated circRNAs in young and senior TB patients. (a) The correlation in Z -scores (computed by a paired Wilcoxon test) between young and senior case-control pairs. Each dot represents one KEGG pathway. The red points stand for the pathways with circular transcripts commonly upregulated in both young and senior TB patients. (b) Paired comparison of circRNA expression in the five prioritized KEGG pathways. (c) Comparison of the fraction of circRNA reads in the five pathways between healthy controls and TB patients.

Article Snippet: The CapitalBio Technology Human CircRNA Array v2 (4 × 180K) was used to quantify circRNA expression.

Techniques: Control, Comparison, Expressing

Journal: EBioMedicine

Article Title: Potential Diagnostic Power of Blood Circular RNA Expression in Active Pulmonary Tuberculosis

doi: 10.1016/j.ebiom.2017.12.007

Figure Lengend Snippet: Technical validation of the five prioritized KEGG pathways using a circRNA expression microarray. Total RNA was treated with RNase R to remove linear transcripts and then subject to microarray hybridization.

Article Snippet: The CapitalBio Technology Human CircRNA Array v2 (4 × 180K) was used to quantify circRNA expression.

Techniques: Biomarker Discovery, Expressing, Microarray, Hybridization

Journal: EBioMedicine

Article Title: Potential Diagnostic Power of Blood Circular RNA Expression in Active Pulmonary Tuberculosis

doi: 10.1016/j.ebiom.2017.12.007

Figure Lengend Snippet: The 7-circRNA signature.

Article Snippet: The CapitalBio Technology Human CircRNA Array v2 (4 × 180K) was used to quantify circRNA expression.

Techniques:

Journal: EBioMedicine

Article Title: Potential Diagnostic Power of Blood Circular RNA Expression in Active Pulmonary Tuberculosis

doi: 10.1016/j.ebiom.2017.12.007

Figure Lengend Snippet: The 7-circRNA signature. (a) Expression heatmap of the circRNAs within the 7-circRNA signature in the discovery cohort. The expression measured by both RNA-seq and circRNA microarray is displayed. (b) Comparison of the expression levels of the 7-circRNA signature between the healthy controls and TB patients in the validation cohort. The error bars represent the standard error of the mean. (c) Principal component analysis of the 7-circRNA signature. PC1: the first principal component; PC2: the second principal component. (d) Comparison of the circRNA-based TB index between the healthy controls and TB patients in the validation cohort. (e) The ROC curve of the TB index in distinguishing TB patients from healthy controls. The light blue area indicates the confidence interval of the ROC curve.

Article Snippet: The CapitalBio Technology Human CircRNA Array v2 (4 × 180K) was used to quantify circRNA expression.

Techniques: Expressing, RNA Sequencing, Microarray, Comparison, Biomarker Discovery